1. Tardigrade
  2. Question
  3. Biology
  4. Fill up the blanks. (A) When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of and these can be joined together for end-to-end using (B) Disruption of the cell wall can be achieved by treating the bacterial cells, plant or animal tissue with enzymes such as (bacteria), (plant cells) and (fungus). (C) If any protein encoding gene is expressed in a heterologous host, it is called as (D) In gel electrophoresis, the separated DNA fragments can be seen only after staining the DNA with a compound known as followed by exposure to UV radiation.

Q. Fill up the blanks.
(A) When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ______ and these can be joined together for end-to-end using
(B) Disruption of the cell wall can be achieved by treating the bacterial cells, plant or animal tissue with enzymes such as______ (bacteria), ______(plant cells) and ____(fungus).
(C) If any protein encoding gene is expressed in a heterologous host, it is called as ______
(D) In gel electrophoresis, the separated DNA fragments can be seen only after staining the DNA with a compound known as______ followed by exposure to UV radiation.

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Solution:

Correct answer is (d) A-sticky ends, DNA ligases; B-lysozyme, cellulase, chitinase; C-recombinant protein; D-ethidium bromide

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