Which statement correctly describes sequential steps in cDNA cloning?

Question

Which statement correctly describes sequential steps in cDNA cloning? (A) reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector (B) mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase,, ligation to vector (C) mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector (D) double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector

Tardigrade Admin · Accepted Answer

Correct answer is (a) reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector

Q. Which statement correctly describes sequential steps in $cD N A$ cloning?

reverse transcription of $m RN A$ , second strand synthesis, $cD N A$ end modification, ligation to vector

$m RN A$ preparation, $cD N A$ synthesis using reverse transcription, second strand synthesis using terminal transferase,, ligation to vector

$m RN A$ synthesis using $RN A$ polymerase, reverse transcription of $m RN A$ , second strand synthesis, ligation to vector

double stranded $cD N A$ synthesis, restriction enzyme digestion, addition of linkers, ligation to vector

Correct answer is (a) reverse transcription of $m RN A$ , second strand synthesis, $cD N A$ end modification, ligation to vector

Q. Which statement correctly describes sequential steps in cDNA cloning?

reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector

mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase,, ligation to vector

mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector

double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector