Question

Which statement correctly describes sequential steps in $cDNA$ cloning?

• A. reverse transcription of $mRNA$, second strand synthesis, $cDNA$ end modification, ligation to vector
• B. $mRNA$ preparation, $cDNA$ synthesis using reverse transcription, second strand synthesis using terminal transferase,, ligation to vector
• C. $mRNA$ synthesis using $RNA$ polymerase, reverse transcription of $mRNA$, second strand synthesis, ligation to vector
• D. double stranded $cDNA$ synthesis, restriction enzyme digestion, addition of linkers, ligation to vector

Answer 1

Answer: (A)

Correct answer is (a) reverse transcription of $mRNA$, second strand synthesis, $cDNA$ end modification, ligation to vector