Question

Which of the following shows the correct sequence of processes with respect to the PCR?

• A. Extension, denaturation, annealing
• B. Denaturation, annealing, extension
• C. Denaturation, extension, annealing
• D. Annealing, extension, denaturation

Answer 1

Answer: (B)

PCR stands for a polymerase chain reaction, in this reaction, multiple copies of the gene of interest are synthesized in vitro using two sets of primers and enzyme $\text{DNA}$ polymerase. The enzyme extends the primers using the nucleotides provided in the reaction and the genomic $\text{DNA}$ as a template. It involves denaturation of the $\text{DNA}$ , attachment of primers called annealing and extension by enzyme $\text{DNA}$ polymerase.