1. Tardigrade
  2. Question
  3. Biology
  4. Read the following statements regarding DNA recombinant technology. Identify the statements that are true and false and select the appropriate option. I. Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. II. Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector. III. The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography. IV. A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection. I II III IV 1 False False True True 2 True True False False 3 True True True True 4 False False True False

Q. Read the following statements regarding DNA recombinant technology. Identify the statements that are true and false and select the appropriate option.

Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.

Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector.

The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography.

A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection.

I II III IV
1 False False True True
2 True True False False
3 True True True True
4 False False True False

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Solution:

A marker is necessary for the DNA recombinant technology to identify the transformed cell which will have our gene of interest. Plasmids are autonomously replicating circular extra-chromosomal DNA hence they are a very good tool for DNA recombination. When gold or tungsten microparticles are used for transferring the gene of interest into the cell, the technique is called gene gun. Micro-injection is the process of injecting a micro-needle into the nucleus to insert the gene of interest. DNA fragments are separated using the process called AGE- agarose gel electrophoresis.