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  4. Read the following statements regarding DNA recombinant technology. Identify the statements that are true and false and select the appropriate option. I. Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. II. Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector. III. The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography. IV. A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection. I II III IV 1 False False True True 2 True True False False 3 True True True True 4 False False True False

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Q. Read the following statements regarding DNA recombinant technology. Identify the statements that are true and false and select the appropriate option.
$I.$ Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
$II.$ Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector.
$III.$ The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography.
$IV.$ A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection.
I II III IV
1 False False True True
2 True True False False
3 True True True True
4 False False True False

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

A marker is necessary for the DNA recombinant technology to identify the transformed cell which will have our gene of interest. Plasmids are autonomously replicating circular extra-chromosomal DNA hence they are a very good tool for DNA recombination. When gold or tungsten microparticles are used for transferring the gene of interest into the cell, the technique is called gene gun. Micro-injection is the process of injecting a micro-needle into the nucleus to insert the gene of interest. DNA fragments are separated using the process called AGE- agarose gel electrophoresis.