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AIPMTAIPMT 2009Biotechnology : Principles and Processes
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Direct gene transfer is the transfer of naked DNA into plant cells, but the presence of rigid plant cell wall acts as a barrier to uptake. Therefore protoplasts are the favoured target for direct gene transfer. Polyethylene glycol mediated DNA uptake is a direct gene transfer method that utilizes the interaction between PEG, naked DNA, salts and the protoplast membrane to effect transport of the DNA into the cytoplasm.