Thank you for reporting, we will resolve it shortly
Q.
Polyethylene glycol method is used for
AIPMTAIPMT 2009Biotechnology : Principles and Processes
Solution:
Direct gene transfer is the transfer of naked DNA into plant cells, but the presence of rigid plant cell wall acts as a barrier to uptake. Therefore protoplasts are the favoured target for direct gene transfer. Polyethylene glycol mediated DNA uptake is a direct gene transfer method that utilizes the interaction between PEG, naked DNA, salts and the protoplast membrane to effect transport of the DNA into the cytoplasm.