Genes express themselves through synthesis of enzyme was demonstrated for the first time in 1941 by G W Beadle and E L Tatum due to their discovery of biochemical mutation in Neurospora. They proposed a concept called one gene-one enzyme hypothesis.
Khorana and Nirenberg cracked the genetic code. They with RW Holley used in vitro system for synthesis of a polypeptide using an artificially synthesized mRNA molecule.
