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  2. Question
  3. Biology
  4. In an experiment, in pBR322 an alien DNA is introduced using Sal I. The transformant will grow on

Q. In an experiment, in pBR322 an alien DNA is introduced using Sal I. The transformant will grow on

 2576  209 NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes Report Error

Solution:

Sal I restriction site is present in Tetracyclin resistant gene. If foreign DNA is inserted in Sal I site than the transformant will loss tetracycline-resistant nature due to insertional inactivation of foreign DNA.