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  4. In an experiment, in pBR322 an alien DNA is introduced using Sal I. The transformant will grow on

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Q. In an experiment, in pBR322 an alien DNA is introduced using Sal I. The transformant will grow on

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

Sal I restriction site is present in Tetracyclin resistant gene. If foreign DNA is inserted in Sal I site than the transformant will loss tetracycline-resistant nature due to insertional inactivation of foreign DNA.