In an experiment, in pBR322 an alien DNA is introduced using Sal I. The transformant will grow on

Question

In an experiment, in pBR322 an alien DNA is introduced using Sal I. The transformant will grow on (A) Tetracycline (B) Ampicillin (C) Both (A) and (B) (D) None of these

Tardigrade Admin · Accepted Answer

Sal I restriction site is present in Tetracyclin resistant gene. If foreign DNA is inserted in Sal I site than the transformant will loss tetracycline-resistant nature due to insertional inactivation of foreign DNA.

Q. In an experiment, in pBR322 an alien DNA is introduced using Sal I. The transformant will grow on

Tetracycline

Ampicillin

Both (A) and (B)

None of these

Sal I restriction site is present in Tetracyclin resistant gene. If foreign DNA is inserted in Sal I site than the transformant will loss tetracycline-resistant nature due to insertional inactivation of foreign DNA.