If pBR322 cloning vector is cut by Pvu I enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants?

Question

If pBR322 cloning vector is cut by Pvu I enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants? (A) ampR gene (B) tetR gene (C) gal gene (D) Both (.A.) and (.B.)

Tardigrade Admin · Accepted Answer

pBR 322 has two selectable markers which are amphicillin and tertacyclin resistant genes. Amphicilin resistant gene has two restriction enzyme recognition site, Pvu I and Pst I. If the vector is cut by Pvu I enzyme for formation of rDNA than amphicillin resistant gene becomes inactive due to insertional inactivation.

Q. If pBR322 cloning vector is cut by Pvu I enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants?

amp^R gene

tet^R gene

gal gene

Both $(A)$ and $(B)$

Q. If pBR322 cloning vector is cut by Pvu I enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants?

ampR gene

tetR gene

gal gene

Both (A) and (B)

amp^R gene

tet^R gene

Both $(A)$ and $(B)$