Q.
If pBR322 cloning vector is cut by Pvu I enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants?
3852
233
NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes
Report Error
Solution:
pBR 322 has two selectable markers which are amphicillin and tertacyclin resistant genes. Amphicilin resistant gene has two restriction enzyme recognition site, Pvu I and Pst I. If the vector is cut by Pvu I enzyme for formation of rDNA than amphicillin resistant gene becomes inactive due to insertional inactivation.