Question

Identify $A$, $B$, $C$ and $D$ in the given figure of $E$. coli doning vector $pBR322$ and select the correct option.

	A	B	C	D
(a)	Hindl	EcoRI	amp${}^R$	ori
(b)	Hindl	BamHl	kan${}^R$	amp${}^R$
(c)	BamHl	Pstl	ori	amp${}^R$
(d)	EcoRI	BamHl	amp${}^R$	ori

• A. a
• B. b
• C. c
• D. d

Answer 1

Answer: (D)

$pBR322$ was the first artificial cloning vector constructed in $1977$ by Boliver and Rodriguez. It is widely used in gene cloning experiments. In $pBR322$ plasmid vector, $p$ - denotes that it is a plasmid; $BR$ - stands for Boliver and Rodriguez who constructed this plasmid; $322$ - Is a number given to distinguish this plasmid from others developed in the same laboratory. $E$. colicloning vector $pBR322$ has estriction sites (Hindlll, EcoRI, BamHl, Sail, PvuW, Pstl, Clal), ori (origin of replication) and antibiotic resistance genes (amp${}^R$ and tet${}^R$). rop codes for the proteins involved in the replication of the plasmid.