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Q. Identify $A$, $B$, $C$ and $D$ in the given figure of $E$. coli doning vector $pBR322$ and select the correct option.

A B C D

(a) Hindl EcoRI amp$^R$ ori

(b) Hindl BamHl kan$^R$ amp$^R$

(c) BamHl Pstl ori amp$^R$

(d) EcoRI BamHl amp$^{R}$ ori

Biotechnology : Principles and Processes

A

a

5%

B

b

4%

C

c

4%

D

d

87%

Solution:

$pBR322$ was the first artificial cloning vector constructed in $1977$ by Boliver and Rodriguez. It is widely used in gene cloning experiments. In $pBR322$ plasmid vector, $p$ - denotes that it is a plasmid; $BR$ - stands for Boliver and Rodriguez who constructed this plasmid; $322$ - Is a number given to distinguish this plasmid from others developed in the same laboratory. $E$. colicloning vector $pBR \,322$ has estriction sites (Hindlll, EcoRI, BamHl, Sail, PvuW, Pstl, Clal), ori (origin of replication) and antibiotic resistance genes (amp$^{R}$ and tet$^R$). rop codes for the proteins involved in the replication of the plasmid.