Q.
Gene of interest was cloned at site Sal I in pBR $322$. The recombinant plasmid will exhibit susceptibility to
2001
192
Biotechnology : Principles and Processes
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Solution:
It has two antibiotic resistance genes (ampR and tet R),
If a gene of interest is cloned at site Sal I in pBR322, the so formed recombinant plasmid will exhibit susceptibility to tetracycline only as tetracycline resistant gene gets inactivated due to insertion.