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  4. Gene of interest was cloned at site Sal I in pBR 322. The recombinant plasmid will exhibit susceptibility to

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Q. Gene of interest was cloned at site Sal I in pBR $322$. The recombinant plasmid will exhibit susceptibility to

Biotechnology : Principles and Processes

Solution:

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It has two antibiotic resistance genes $\left(a m p^{R}\right.$ and tet $\left.^{R}\right)$,
If a gene of interest is cloned at site Sal I in pBR$322$, the so formed recombinant plasmid will exhibit susceptibility to tetracycline only as tetracycline resistant gene gets inactivated due to insertion.