Question

Enzymes that catalyse the substrate level phosphorylation reaction in glycolysis belong to

• A. oxidoreductases
• B. transferases
• C. hydrolases
• D. ligases

Answer 1

Answer: (B)

In glycolysis, substrate level phosphorylation occurs in two steps :

- Formation of 3-phosphoglycerate from 1, 3-bisphosphoglycerate catalysed by enzyme phosphoglycerate kinase.

- Formation of pyruvate from phosphoenol pyruvate catalysed by enzyme pyruvate kinase.

Both these enzymes belong to the category of transferases. They transfer phosphate group from ATP to a substrate.