DNA is a polymer of nucleotides which are linked to each other by 3'-5' phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose?

Question

DNA is a polymer of nucleotides which are linked to each other by 3'-5' phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose? (A) Replace purines with pyrimidines (B) Remove/Replace 3' OH group in deoxyribose (C) Remove/Replace 2' OH group with some other group in deoxyribose (D) Both (b) and (c)

Tardigrade Admin · Accepted Answer

If 3' OH group is removed/replaced in deoxyribose, there will be no formation of phosphodiester bonds and hence polymerisation of nucleotides will be prevented.

Q. $D N A$ is a polymer of nucleotides which are linked to each other by $3^{'} - 5^{'}$ phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose?

Replace purines with pyrimidines

Remove/Replace $3^{'} O H$ group in deoxyribose

Remove/Replace $2^{'} O H$ group with some other group in deoxyribose

Both (b) and (c)

If $3^{'} O H$ group is removed/replaced in deoxyribose, there will be no formation of phosphodiester bonds and hence polymerisation of nucleotides will be prevented.

Q. DNA is a polymer of nucleotides which are linked to each other by 3′−5′ phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose?

Replace purines with pyrimidines

Remove/Replace 3′OH group in deoxyribose

Remove/Replace 2′OH group with some other group in deoxyribose

Both (b) and (c)

If 3′OH group is removed/replaced in deoxyribose, there will be no formation of phosphodiester bonds and hence polymerisation of nucleotides will be prevented.

Q. $D N A$ is a polymer of nucleotides which are linked to each other by $3^{'} - 5^{'}$ phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose?

Remove/Replace $3^{'} O H$ group in deoxyribose

Remove/Replace $2^{'} O H$ group with some other group in deoxyribose

If $3^{'} O H$ group is removed/replaced in deoxyribose, there will be no formation of phosphodiester bonds and hence polymerisation of nucleotides will be prevented.