Consider the linear double-stranded DNA shown below. The restriction enzymes sites and the lengths demarcated are shown. This DNA is completely digested with both EcoRI and BamHl restriction enzymes. If the product is analysed by gel electrophoresis, how many distinct bands would be observed?

Question

Consider the linear double-stranded DNA shown below. The restriction enzymes sites and the lengths demarcated are shown. This DNA is completely digested with both EcoRI and BamHl restriction enzymes. If the product is analysed by gel electrophoresis, how many distinct bands would be observed? <img class="img-fluid question-image" alt="image" src="https://cdn.tardigrade.in/img/question/biology/ac616dbd08295d294a8aaf25471674eb-.png" /> (A) 5 (B) 2 (C) 3 (D) 4

Tardigrade Admin · Accepted Answer

The gel electrophoresis will show 3 bands because even though 4 fragments are formed but 2 fragments are of 3 kb and they will appear as one single band. <img class="img-fluid question-image" alt="image" src="https://cdn.tardigrade.in/img/question/biology/48767065d2767715246bdcbd8aa51663-.png" />

Q. Consider the linear double-stranded DNA shown below. The restriction enzymes sites and the lengths demarcated are shown. This DNA is completely digested with both EcoRI and BamHl restriction enzymes. If the product is analysed by gel electrophoresis, how many distinct bands would be observed?

$5$

$2$

$3$

$4$

The gel electrophoresis will show $3$ bands because even though $4$ fragments are formed but $2$ fragments are of $3$ kb and they will appear as one single band.