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  4. Consider the linear double-stranded DNA shown below. The restriction enzymes sites and the lengths demarcated are shown. This DNA is completely digested with both EcoRI and BamHl restriction enzymes. If the product is analysed by gel electrophoresis, how many distinct bands would be observed? <img class=img-fluid question-image alt=image src=https://cdn.tardigrade.in/img/question/biology/ac616dbd08295d294a8aaf25471674eb-.png />

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Q. Consider the linear double-stranded DNA shown below. The restriction enzymes sites and the lengths demarcated are shown. This DNA is completely digested with both EcoRI and BamHl restriction enzymes. If the product is analysed by gel electrophoresis, how many distinct bands would be observed?
image

KVPYKVPY 2015

Solution:

The gel electrophoresis will show $3$ bands because even though $4$ fragments are formed but $2$ fragments are of $3$ kb and they will appear as one single band.
image