Consider the following two statements and select the correct option. Statement I: Unless one cuts the vector and the foreign/ source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created. Statement II: When cut by different restriction enzymes, the resultant DNA fragments have the same kind of sticky ends which can be joined together (end to end) using DNA ligases.

Question

Consider the following two statements and select the correct option. Statement I: Unless one cuts the vector and the foreign/ source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created. Statement II: When cut by different restriction enzymes, the resultant DNA fragments have the same kind of sticky ends which can be joined together (end to end) using DNA ligases. (A) Only statement I is correct (B) Only statement II is correct (C) Both statements I and II are correct (D) Both statements I and II are incorrect

Tardigrade Admin · Accepted Answer

Unless one cuts the vector and the foreign/source DNA with, the same restriction enzyme, the recombinant vector molecule cannot be created because when cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases.