1. Tardigrade
  2. Question
  3. Biology
  4. Consider the following two statements and select the correct option. Statement I: Unless one cuts the vector and the foreign/ source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created. Statement II: When cut by different restriction enzymes, the resultant DNA fragments have the same kind of sticky ends which can be joined together (end to end) using DNA ligases.

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Q. Consider the following two statements and select the correct option.
Statement I : Unless one cuts the vector and the foreign/ source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created.
Statement II: When cut by different restriction enzymes, the resultant DNA fragments have the same kind of sticky ends which can be joined together (end to end) using DNA ligases.

Biotechnology : Principles and Processes

Solution:

Unless one cuts the vector and the foreign/source DNA with, the same restriction enzyme, the recombinant vector molecule cannot be created because when cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases.