Q. Assume the thylakoid membrane within a chloroplast is punctured so that there is no separation between lumen & stroma of thylakoid. Which of these process would be most affected?

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Solution:

According to chemiosmotic hypothesis (Mitchell 1961 ) the ATP is synthesized on ATPase complexes located on the non appressed portions of thylakoid membranes particularly towards margins. Therefore, if the thylakoid membrane within a chloroplast is punctured so that there is no separation between lumen and stroma of thylakoid, synthesis of ATP will be affected the most.