Question

Assume the thylakoid membrane within a chloroplast is punctured so that there is no separation between lumen & stroma of thylakoid. Which of these process would be most affected?

• A. the splitting of water
• B. the synthesis of ATP
• C. reduction of $NADP ^{+}$
• D. the flow of electrons from PS - II to PS - I

Answer 1

Answer: (B)

According to chemiosmotic hypothesis (Mitchell 1961 ) the ATP is synthesized on ATPase complexes located on the non appressed portions of thylakoid membranes particularly towards margins. Therefore, if the thylakoid membrane within a chloroplast is punctured so that there is no separation between lumen and stroma of thylakoid, synthesis of ATP will be affected the most.