Assertion: Use of chitinase enzyme is necessary for isolation of DNA % from yeast cells but not in case of Spirogyra. Reason: Fungal cell wall is made up of fungal cellulose or chitin.

Question

Assertion: Use of chitinase enzyme is necessary for isolation of DNA % from yeast cells but not in case of Spirogyra. Reason: Fungal cell wall is made up of fungal cellulose or chitin. (A) If both assertion and reason are true and reason is the correct explanation of assertion (B) If both assertion and reason are true but reason is not the correct explanation of assertion (C) If assertion is true but reason is false (D) If both assertion and reason are false

Tardigrade Admin · Accepted Answer

Release of DNA from a cell is achieved by treating the cells or tissues with enzymes such as lysozyme (bacteria), cellulose (plant cells), chitinase (fungus), etc. These enzymes degrade cell wall; plasma membrane degrading enzymes like lipase, etc., are also needed. Since yeast is a fungus and fungal cell wall is made of chitin (fungal cellulose), isolation of DNA necessarily requires use of enzyme chitinase.

Q. Assertion : Use of chitinase enzyme is necessary for isolation of DNA% from yeast cells but not in case of Spirogyra. Reason : Fungal cell wall is made up of fungal cellulose or chitin.

If both assertion and reason are true and reason is the correct explanation of assertion

If both assertion and reason are true but reason is not the correct explanation of assertion

If assertion is true but reason is false

If both assertion and reason are false

Q. Assertion : Use of chitinase enzyme is necessary for isolation of $D N A %$ from yeast cells but not in case of Spirogyra.
Reason : Fungal cell wall is made up of fungal cellulose or chitin.