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  4. Assertion: Use of chitinase enzyme is necessary for isolation of DNA % from yeast cells but not in case of Spirogyra. Reason: Fungal cell wall is made up of fungal cellulose or chitin.

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Q. Assertion : Use of chitinase enzyme is necessary for isolation of $DNA\%$ from yeast cells but not in case of Spirogyra.
Reason : Fungal cell wall is made up of fungal cellulose or chitin.

Biotechnology : Principles and Processes

Solution:

Release of $DNA$ from a cell is achieved by treating the cells or tissues with enzymes such as lysozyme (bacteria), cellulose (plant cells), chitinase (fungus), etc. These enzymes degrade cell wall; plasma membrane degrading enzymes like lipase, etc., are also needed. Since yeast is a fungus and fungal cell wall is made of chitin (fungal cellulose), isolation of $DNA$ necessarily requires use of enzyme chitinase.