Assertion: E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. Reason: Recognition site for BamHI is present in tetR region of pBR322.

Question

Assertion: E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. Reason: Recognition site for BamHI is present in tetR region of pBR322. (A) If both assertion and reason are true and reason is the correct explanation of assertion (B) If both assertion and reason are true but reason is not the correct explanation of assertion (C) If assertion is true but reason is false (D) If both assertion and reason are false

Tardigrade Admin · Accepted Answer

pBR322 has recognition sites for several commonly used restriction enzymes. Recognition site for BamHI is present in tetr region i.e., region responsible for tetracycline resistance. When an insert is added at the BamHI recognition site the gene for tetracycline resistance becomes non-functional and the recombinant bacteria with plasmid pBR322 that has DNA insert at BamHI lose tetracycline resistance.

Q. Assertion : E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. Reason : Recognition site for BamHI is present in tetR region of pBR322.

If both assertion and reason are true and reason is the correct explanation of assertion

If both assertion and reason are true but reason is not the correct explanation of assertion

If assertion is true but reason is false

If both assertion and reason are false

Q. Assertion : E.coli having $pBR 322$ with $D N A$ insert at BamHI site cannot grow in medium containing tetracycline.
Reason : Recognition site for BamHI is present in $t e t^{R}$ region of $pBR 322$ .