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  4. Assertion: E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. Reason: Recognition site for BamHI is present in tetR region of pBR322.

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Q. Assertion : E.coli having $pBR322$ with $DNA$ insert at BamHI site cannot grow in medium containing tetracycline.
Reason : Recognition site for BamHI is present in $tet^R$ region of $pBR322$.

Biotechnology : Principles and Processes

Solution:

$pBR322$ has recognition sites for several commonly used restriction enzymes. Recognition site for BamHI is present in tetr region i.e., region responsible for tetracycline resistance. When an insert is added at the BamHI recognition site the gene for tetracycline resistance becomes non-functional and the recombinant bacteria with plasmid $pBR322$ that has $DNA$ insert at BamHI lose tetracycline resistance.