Question

Assertion (A) A genetic probe is helpful in the detection of specific DNA sequence.
Reason (R) Genetic probe is a radiolabelled C-DNA, which has complementary base sequence, to DNA fragment which is to be detected.

• A. Both Assertion and Reason are true and Reason is the correct explanation of Assertion
• B. Both Assertion and Reason are true, but Reason is not the correct explanation of Assertion
• C. Assertion is true, but Reason is false
• D. Both Assertion and Reason are false

Answer 1

Answer: (A)

Probes are small nucleotide sequences used to detect the presence of complementary sequences in nucleic acid. By permitting the probes to base pair with the sample nucleic acid and then identifying the samples that show base pairing with probes, we can detect required nucleotide sequence. These probes can be labelled either with radioactive substances (like $P^{32},C^{14}$ ) or with non-radioactive substances (like biotin, digoxigenin).