A mixture contains three amino acids A (pH = 3.2), B (pH = 5.7) and C (pH = 9.7). When it is subjected to electrophoresis at pH 7.7, in which direction will each component of the mixture move?

Question

A mixture contains three amino acids A (pH = 3.2), B (pH = 5.7) and C (pH = 9.7). When it is subjected to electrophoresis at pH 7.7, in which direction will each component of the mixture move? (A) A to anode, B and C to cathode (B) A to anode, B no movement, C to cathode (C) A and B to anode and C to cathode (D) A to cathode, B no movement, C to anode

Tardigrade Admin · Accepted Answer

Since pH of A and B is less than pH (7.7), so they will exist in anionic form and will migrate towards anode. Since pH of C is greater than the pH of medium, so it will exist in cationic form and migrate towards cathode

Q. A mixture contains three amino acids $A (p H = 3.2), B (p H = 5.7)$ and $C (p H = 9.7)$ .
When it is subjected to electrophoresis at $p H 7.7$ , in which direction will each component of the mixture move?

$A$ to anode, $B$ and $C$ to cathode

$A$ to anode, $B$ no movement, $C$ to cathode

$A$ and $B$ to anode and $C$ to cathode

$A$ to cathode, $B$ no movement, $C$ to anode

Since $p H$ of $A$ and $B$ is less than $p H (7.7)$ , so they will exist in anionic form and will migrate towards anode.
Since $p H$ of $C$ is greater than the $p H$ of medium, so it will exist in cationic form and migrate towards cathode

Q. A mixture contains three amino acids A(pH=3.2),B(pH=5.7) and C(pH=9.7). When it is subjected to electrophoresis at pH7.7, in which direction will each component of the mixture move?

A to anode, B and C to cathode

A to anode, B no movement, C to cathode

A and B to anode and C to cathode

A to cathode, B no movement, C to anode