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Q. A mixture contains three amino acids $A (pH = 3.2), B (pH = 5.7)$ and $C (pH = 9.7)$.
When it is subjected to electrophoresis at $pH \,7.7$, in which direction will each component of the mixture move?

Biomolecules

Solution:

Since $pH$ of $A$ and $B$ is less than $pH (7.7)$, so they will exist in anionic form and will migrate towards anode.
Since $pH$ of $C$ is greater than the $pH$ of medium, so it will exist in cationic form and migrate towards cathode