A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be

Question

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be (A) Human gene may have intron which bacteria cannot process (B) Amino acid codons for humans and bacteria are different (C) Human protein is formed but degraded by bacteria (D) All the above

Tardigrade Admin · Accepted Answer

Eukaryotic genes do not function properly when transferred into bacterial cell because introns are present in eukaryotic cells but are absent in prokaryotic cells. Hence, when bacterial cell is transformed with recombinant DNA, which is generated using human gene, it could not process it. As a result, no desired protein will be produced.

Q. A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be

Human gene may have intron which bacteria cannot process

Amino acid codons for humans and bacteria are different

Human protein is formed but degraded by bacteria

All the above