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  4. A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be

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Q. A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be

Biotechnology : Principles and Processes

Solution:

Eukaryotic genes do not function properly when transferred into bacterial cell because introns are present in eukaryotic cells but are absent in prokaryotic cells. Hence, when bacterial cell is transformed with recombinant DNA, which is generated using human gene, it could not process it. As a result, no desired protein will be produced.