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Q.
Which of the following companies started selling humulin in the year $1983$?
Biotechnology and its Applications
Solution:
In $1983$, Eli Lilly an American company, first prepared two $DNA$ sequences corresponding to $A$ and $B$ chains of human insulin and introduced them in plasmids of Escherichia coli to produce insulin chains. Chains $A$ and $B$ were produced separately, extracted and combined by creating disulphide bonds to form human insulin (humulin).