Q. With regard to the proton gradient associated with chloroplasts, consider the following statements:
(1) Within the chloroplast, protons in the stroma increase in number than the lumen.
(2) The predient is broken down due to the movement of electrons across the membrane.
Which of the statement(s) given above is/are correct?

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Solution:

• Within the chloroplast, protons in the stroma decrease in number, while in the lumen there is accumulation of protons. This creates a proton gradient across the thylakoid membrane as well as a measurable decrease in pH in the lumen.
• The breakdown of proton gradient leads to release of energy.
• The gradient is broken down due to the movement of protons across the membrane to the stroma through the trans-membrane channel of the F0 of the ATPase.