Which type of primer is used the genetic engineering tool -Polymerase Chain Reaction?

Question

Which type of primer is used the genetic engineering tool -Polymerase Chain Reaction? (A) Complementary to 5 prime end of DNA template. (B) Complementary to 3 prime end of DNA template. (C) Complementary to both 3 prime and 5 prime end of DNA template. (D) Complementary to each other.

Tardigrade Admin · Accepted Answer

The Taq polymerase enzyme that adds up and extends the primer follows the 5’ arrow 3’ direction for the new strand made based on the template. The template strand is the complementary of new strand/primer whose direction is opposite - 3’ arrow 5’ .

Q. Which type of primer is used the genetic engineering tool -Polymerase Chain Reaction?

Complementary to $5^{'}$ end of DNA template.

Complementary to $3^{'}$ end of DNA template.

Complementary to both $3^{'}$ and $5^{'}$ end of DNA template.

Complementary to each other.

The Taq polymerase enzyme that adds up and extends the primer follows the $5’ \to 3’$ direction for the new strand made based on the template. The template strand is the complementary of new strand/primer whose direction is opposite - $3’ \to 5’$ .

Q. Which type of primer is used the genetic engineering tool -Polymerase Chain Reaction?

Complementary to 5′ end of DNA template.

Complementary to 3′ end of DNA template.

Complementary to both 3′ and 5′ end of DNA template.

Complementary to each other.

The Taq polymerase enzyme that adds up and extends the primer follows the 5’→3’ direction for the new strand made based on the template. The template strand is the complementary of new strand/primer whose direction is opposite - 3’→5’ .

Complementary to $5^{'}$ end of DNA template.

Complementary to $3^{'}$ end of DNA template.

Complementary to both $3^{'}$ and $5^{'}$ end of DNA template.

The Taq polymerase enzyme that adds up and extends the primer follows the $5’ \to 3’$ direction for the new strand made based on the template. The template strand is the complementary of new strand/primer whose direction is opposite - $3’ \to 5’$ .