Which part of the plasmid pBR322 is linked to the part that controls copy number of foreign DNA?

Question

Which part of the plasmid pBR322 is linked to the part that controls copy number of foreign DNA? (A) Multiple restriction site (B) Multiple cloning sites (C) Selectable marker (D) Origin of replication

Tardigrade Admin · Accepted Answer

The nucleotide sequence of 'Ori' is also responsible for controlling the copy number of the plasmid and linked DNA. So, in order to obtain multiple copies of desired DNA, it should be cloned in a vector whose origin supports high copy number.

Q. Which part of the plasmid pBR322 is linked to the part that controls copy number of foreign DNA?

Multiple restriction site

Multiple cloning sites

Selectable marker

Origin of replication

The nucleotide sequence of 'Ori' is also responsible for controlling the copy number of the plasmid and linked DNA. So, in order to obtain multiple copies of desired DNA, it should be cloned in a vector whose origin supports high copy number.