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Question
Biology
Which part of the plasmid pBR322 is linked to the part that controls copy number of foreign DNA?
Q. Which part of the plasmid pBR322 is linked to the part that controls copy number of foreign DNA?
196
181
NTA Abhyas
NTA Abhyas 2020
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A
Multiple restriction site
B
Multiple cloning sites
C
Selectable marker
D
Origin of replication
Solution:
The nucleotide sequence of 'Ori' is also responsible for controlling the copy number of the plasmid and linked DNA. So, in order to obtain multiple copies of desired DNA, it should be cloned in a vector whose origin supports high copy number.