Q. Which one of the following is not essential for Polymerase Chain Reaction (PCR) ?

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Solution:

For PCR, there are five chemical components needed, including a DNA template, DNA polymerase enzyme, primers, nucleotides and reaction buffer. The Polymerase Chain Reaction (PCR) is used to make millions of copies of a target piece of DNA. Thus, option (a) is correct as restriction enzyme is used for recombinant DNA technology,