Q.
Which one of the following equations suggests that O2 released during photosynthesis comes from water?
2342
208
Photosynthesis in Higher Plants
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Solution:
Ruben and Kamen (1941), while working on chlorella (unicellular green alga ) found that oxygen liberated during photosynthesis comes from water.
(i) When normal H2O radioactive CO2 (i.e. CO218 ) were used, normal O2 is evolved. nCO218+nH2O Light Chlorophyll (CH2O18)n+nO2↑( normal )+H2O
(ii) When normal CO2 radioactive H2O( i.e. H2O18) were used, radioactive O2( i.e. O218) is evolved. nCO2+nH2O18→(CH2O)n+O218↑ (radioactive oxygen) +H2O