Question

Which of the following statement is correct about autoradiography?

• A. A double-stranded DNA or RNA probe is allowed to hybridize to its complementary DNA.
• B. The clone having the mutated gene will appear on the photographic film.
• C. The probe will not have complementarity with the mutated gene.
• D. More than one option is correct

Answer 1

Answer: (C)

A single-stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridize to its complementary DNA in a clone of cells followed by detection using autoradiography. The clone having the mutated gene will hence not appear on the photographic film, because the probe will not have complementarity with the mutated gene.