Question

Which of the following could be a restriction enzyme recognition site?

• A. ATGCAT
• B. ATCATC
• C. AAGGA
• D. ATCCTA

Answer 1

Answer: (A)

r-DNA technology is a process that uses endonucleases for making transformants. In it, a specific part of DNA sequence is either removed or added. The endonucleases make cut at a specific sequence of the DNA called a recognition site. These sequences are palindromic in nature. Palindromic sequence have the same sequence of base pairs on both strands when read from both the direction $-3’\to 5’or5’\to 3’.$
The sequence ATGCAT will have the complementary palindromic base sequence as TACGAT.