Which of the following can distinguish transformants from non-transformants?

Question

Which of the following can distinguish transformants from non-transformants? (A) Presence of more than one recognition site in the vector DNA (B) Presence of foreign DNA in the vector DNA results in insertional inactivation of selectable markers (C) Antibiotic-resistance gene gets inactivated due to insertion of foreign DNA (D) Both 2 and 3

Tardigrade Admin · Accepted Answer

Selectable markers help in detecting and selectively permitting the growth of the desired transformants.

Insertional inactivation is a technique where foreign DNA is inserted into a restriction site inside an antibiotic resistance gene causing the gene to get inactivated.

Q. Which of the following can distinguish transformants from non-transformants?

Presence of more than one recognition site in the vector DNA

Presence of foreign DNA in the vector DNA results in insertional inactivation of selectable markers

Antibiotic-resistance gene gets inactivated due to insertion of foreign DNA

Both 2 and 3

Selectable markers help in detecting and selectively permitting the growth of the desired transformants.

Insertional inactivation is a technique where foreign DNA is inserted into a restriction site inside an antibiotic resistance gene causing the gene to get inactivated.