Cellulase and pectinase enzymes are required to obtains naked protoplasts during somatic hybridization.
When a hybrid is produced by fusion of somatic cells of two varieties or species, it is known as somatic hybrid. The process of producing somatic hybrids is called somatic hybridization. First, the cell wall of the plant cells are removed by digestion with a combination of pectinase and cellulase. The plant cells without cell wall are called protoplasts.