Q. The unequivocal proof that DNA is the genetic material came from the experiment of ____
Solution:
Alfred Hershey and Martha Chase experiment was the first experiment by which all biologists were convinced that DNA is the genetic material. They worked with T2 bacteriophage that infects bacteria E. coli.
In his experiment, they used two radioisotopes 32P (in DNA) and 35S (in protein) in two different types of phages and then are allowed to infect E.coli separately. Then both types of cultures are centrifuged.
After centrifugation, it was found that phage having radioactive DNA was found in the bacterial cell (in the sediment), this indicates that DNA has been injected into the bacterial cell.
Miescher discovered nucleic acid from pus cells and named it Nuclein.
F. Griffith performed the transformation experiment using bacteria Streptococcus pneumoniae which causes pneumonia.
Watson and Crick gave double helix model of B-DNA.
In his experiment, they used two radioisotopes 32P (in DNA) and 35S (in protein) in two different types of phages and then are allowed to infect E.coli separately. Then both types of cultures are centrifuged.
After centrifugation, it was found that phage having radioactive DNA was found in the bacterial cell (in the sediment), this indicates that DNA has been injected into the bacterial cell.
F. Griffith performed the transformation experiment using bacteria Streptococcus pneumoniae which causes pneumonia.
Watson and Crick gave double helix model of B-DNA.