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  4. The sticky ends on each of the two strands of a DNA generated by treatment with restriction enzymes facilitate the action of enzyme

Q. The sticky ends on each of the two strands of a DNA generated by treatment with restriction enzymes facilitate the action of enzyme

 2398  223 AMUAMU 2014Molecular Basis of Inheritance Report Error

Solution:

Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strands. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase,