Question

The restriction endonucleases are utilised in genetic engineering as

• A. Molecular cement for combining DNA fragments into long chain.
• B. Tools of recombinant DNA technology.
• C. Molecular scalpels for cutting DNA at specific sites.
• D. Both (B) and (C)

Answer 1

Answer: (D)

Restriction enzyme or restrictase or endonuclease is an enzyme that cleaves DNA into pieces or fragments at or near specific sites called restriction sites. GAATTC is the recognition sequence for the enzyme EcoRI.
They are extensively used in recombinant DNA technology and are often known as molecular scissors.