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  4. The main cloning vectors used for DNA sequencing in the Human Genome Project are .

Q. The main cloning vectors used for DNA sequencing in the Human Genome Project are ________________.

 2041  237 NTA AbhyasNTA Abhyas 2020Molecular Basis of Inheritance Report Error

Solution:

For sequence annotation in HGP, the total DNA from a cell is isolated and converted into random fragments of relatively smaller sizes (recall DNA is a very long polymer, and there are technical limitations in sequencing very long pieces of DNA) and cloned in suitable host using specialised vectors. The cloning results in the amplification of each piece of DNA fragment so that it could be subsequently sequenced with ease. The commonly used hosts were bacteria and yeast, and the vectors were called BAC (bacterial artificial chromosomes) and YAC (yeast artificial chromosomes), respectively.